rat anti mouse cd3 primary antibody  (Bio-Rad)

Journal: bioRxiv

Article Title: Multimodal Profiling of Repair-associated Immune Dynamics in a Mouse Model of Menstruation

doi: 10.64898/2026.02.06.704418

Figure Lengend Snippet: (A) Population restricted FC analysis to identify CD45 + Ly6G − NK1.1 − CD19 − CD3 + T cells in uterine tissue digests. (B) Bar plot, FC quantification: Relative abundance of T cells calculated as a percentage of total CD45 + cells per uterine horn. (C) Bar plot, FC quantification: Absolute counts of T cells per uterine horn. (D) Immunohistochemistry: detection of CD3 + T cells (black arrows) in uterine tissues 24- and 48 hr after progesterone withdrawal (representative images: 24 hr n=5, 48 hr n=6, scale bar= 50µm). (E) Population restricted FC analysis to identify CD45 + Ly6G − CD3 − CD19 − NK1.1 + NK cells in uterine tissue digests. (F) Bar plot, FC quantification: Relative abundance of NK cells calculated as a percentage of total CD45 + cells per uterine horn. (G) Bar plot, FC quantification: Absolute counts of NK cells per uterine horn. (H) Immunohistochemistry: detection of DBA-bound NK cells in uterine tissues 24- and 48 hr after progesterone withdrawal (representative images: 24 hr n=4, 48 hr n=4, scale bar= 50µm). (I) Population restricted FC analysis to identify CD45 + Ly6G − CD3 − NK1.1 − CD19 + B cells in uterine tissue digests (J) Bar plot, FC quantification: Relative abundance of B cells calculated as a percentage of total CD45 + cells per uterine horn. (K) Bar plot, FC quantification: Absolute counts of B cells per uterine horn. (L) Immunohistochemistry: detection of B220 + B cells (black arrows) in uterine tissues 24- and 48 hr after progesterone withdrawal (representative images: 24 hr n=4, 48 hr n=4, scale bar= 50µm). (FC analysis groups: control (n=15), 0 hr (prior to breakdown, n=17), 12 hr (tissue breakdown, n=22), 24 hr (tissue repair, n=16) and 48 hr (tissue remodelling, n=10) after progesterone withdrawal; statistical comparisons were made using Kruskal-Wallis tests with multiple comparisons. * p< 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

Article Snippet: Primary antibodies against CD3 (1:250 dilution; NB600-1441, Novus Biologicals), B220 (1:250 dilution; 14-0452-82, Invitrogen), CD14 (1:6000 dilution; ab221678, Abcam), CD64 (1:6000 dilution; 50086-R008, Sino Biological), Ly6G (1:10000 dilution; 127649, Biolegend) and DBA-lectin (1:1000 dilution; B-1035, Vector) were used in this study.

Techniques: Immunohistochemistry, Control

Journal: bioRxiv

Article Title: Multimodal Profiling of Repair-associated Immune Dynamics in a Mouse Model of Menstruation

doi: 10.64898/2026.02.06.704418

Figure Lengend Snippet: Immunohistochemistry: detection of (A) CD3+ T cells, (B) DBA-lectin+ NK cells and (C) B220+ B cells in uterine tissue sections at 0hr (prior to breakdown, n=4), 12hr (tissue breakdown, n=4-6), 24hr (tissue repair, n=4-5) and 48hr (tissue remodelling, n=4-6) hr after progesterone withdrawal (representative images, scale bar=500µm).

Article Snippet: Primary antibodies against CD3 (1:250 dilution; NB600-1441, Novus Biologicals), B220 (1:250 dilution; 14-0452-82, Invitrogen), CD14 (1:6000 dilution; ab221678, Abcam), CD64 (1:6000 dilution; 50086-R008, Sino Biological), Ly6G (1:10000 dilution; 127649, Biolegend) and DBA-lectin (1:1000 dilution; B-1035, Vector) were used in this study.

Techniques: Immunohistochemistry

Journal: bioRxiv

Article Title: Multimodal Profiling of Repair-associated Immune Dynamics in a Mouse Model of Menstruation

doi: 10.64898/2026.02.06.704418

Figure Lengend Snippet: (A) Population restricted FC analysis to identify CD45 + CD3 − CD19 − NK1.1 − CD11b + Ly6G − SiglecF − CD64 − Ly6C + MHCII − monocytes in uterine tissue digests. (B) Bar plot, FC quantification: Relative abundance of monocytes calculated as a percentage of total CD45 + cells per uterine horn. (C) Bar plot, FC quantification: Absolute counts of monocytes per uterine horn. (D) Population restricted FC analysis of CD45 + CD3 − CD19 − NK1.1 − CD11b + Ly6G − SiglecF − CD64 + Ly6C − F4/80 + MHCII + macrophages in uterine tissue digests. (E) Bar plot, FC quantification: Relative abundance of macrophages calculated as a percentage of total CD45 + cells per uterine horn. (F) Bar plot, FC quantification: Absolute counts of macrophages per uterine horn. (G) Population restricted FC analysis of CD45 + CD3 − CD19 − NK1.1 − CD11b + Ly6G + SiglecF − neutrophils in uterine tissue digests. (H) Bar plot, FC quantification: Relative abundance of neutrophils calculated as a percentage of total CD45 + cells per uterine horn. (I) Bar plot, FC quantification: Absolute counts of neutrophils per uterine horn. (FC analysis groups: control (n=15), 0 hr (prior to breakdown, n=17), 12 hr (tissue breakdown, n=22), 24 hr (tissue repair, n=16) and 48 hr (tissue remodelling, n=10) after progesterone withdrawal; statistical comparisons were made using Kruskal-Wallis tests with multiple comparisons. * p< 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). (J-K) Immunohistochemical detection of CD14 + monocytes (cyan), CD64 + macrophages (yellow) and Ly6G + neutrophils (magenta) (merged and split channel) in uterine tissue cross sections at (J) 24 hr (repair, n=5) and (K) 48 hr (remodelling, n=3) following progesterone withdrawal (merged and split channel representative images, scale bar = 1000µm).

Article Snippet: Primary antibodies against CD3 (1:250 dilution; NB600-1441, Novus Biologicals), B220 (1:250 dilution; 14-0452-82, Invitrogen), CD14 (1:6000 dilution; ab221678, Abcam), CD64 (1:6000 dilution; 50086-R008, Sino Biological), Ly6G (1:10000 dilution; 127649, Biolegend) and DBA-lectin (1:1000 dilution; B-1035, Vector) were used in this study.

Techniques: Control, Immunohistochemical staining

Journal: bioRxiv

Article Title: Multimodal Profiling of Repair-associated Immune Dynamics in a Mouse Model of Menstruation

doi: 10.64898/2026.02.06.704418

Figure Lengend Snippet: (A) Population restricted FC analysis to identify CD45+ CD3- CD19- NK1.1- Ly6G- CD64- CD11c+ MHCII+ dendritic cells (DCs) in uterine tissue digests. (B) Bar plot, FC quantification: Relative abundance of DCs calculated as a percentage of total CD45+ cells per uterine horn. (C) Bar plot, FC quantification: Absolute counts of DCs per uterine horn. (D) Population restricted FC analysis to CD45+ CD3- CD19- NK1.1- CD11b+ Ly6G- SiglecF+ eosinophils in uterine tissue digests. (E) Bar plot, FC quantification: Relative abundance of eosinophils calculated as a percentage of total CD45+ cells per uterine horn. (F) Bar plot, FC quantification: Absolute counts of eosinophils per uterine horn. (FC analysis groups: control (n=15), 0hr (prior to breakdown, n=17), 12hr (tissue breakdown, n=22), 24hr (tissue repair, n=16) and 48hr (tissue remodelling, n=10) after progesterone withdrawal; statistical comparisons were made using Kruskal-Wallis tests with multiple comparisons. and * p< 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

Article Snippet: Primary antibodies against CD3 (1:250 dilution; NB600-1441, Novus Biologicals), B220 (1:250 dilution; 14-0452-82, Invitrogen), CD14 (1:6000 dilution; ab221678, Abcam), CD64 (1:6000 dilution; 50086-R008, Sino Biological), Ly6G (1:10000 dilution; 127649, Biolegend) and DBA-lectin (1:1000 dilution; B-1035, Vector) were used in this study.

Techniques: Control

Journal: ImmunoTargets and Therapy

Article Title: Enhancing Anti-CTLA-4 Antibody Delivery to the Brain Using Focused Ultrasound and Microbubbles

doi: 10.2147/ITT.S569804

Figure Lengend Snippet: Co-localisation of CD3 + cells and MHC class II + cells with anti-CTLA-4 antibody . ( A and B ) Immunofluorescence staining of CD3 + cells in long-pulse ultrasound treated brains ( A ) and RaSP-treated brains ( B ) of wild-type mice superimposed with anti-CTLA-4 antibody fluorescence and DAPI (nuclei staining). ( C and D ) Immunofluorescence staining of MHC class II + cells in long-pulse ( C ) or RaSP-treated brains ( D ) of wild-type mice superimposed with anti-CTLA-4 antibody fluorescence and DAPI (nuclei staining).

Article Snippet: T cells were stained using a rat anti-mouse CD3 primary antibody (1:200; clone: KT3, #MCA500G, Bio-Rad, CA, USA) with 1% (w/v) milk powder (Tesco, Hertfordshire, England) dissolved in TBS (#524750-1EA, EMD Millipore Corporation, Burlington, MA, USA) and a secondary Alexa Fluor 488 goat anti-rat IgG H&L secondary antibody (1:400; #ab150157, Abcam, Cambridge, England) with 1% (w/v) milk powder in TBS.

Techniques: Immunofluorescence, Staining, Fluorescence

Journal: ImmunoTargets and Therapy

Article Title: Enhancing Anti-CTLA-4 Antibody Delivery to the Brain Using Focused Ultrasound and Microbubbles

doi: 10.2147/ITT.S569804

Figure Lengend Snippet: CD3 + and MHC class II + invasion in ultrasound-treated brain regions . Immunofluorescence staining shows differences in the invasion of CD3 + ( A – D ) and MHC class II + ( E – H ) cells into the ultrasound-treated brain regions ( A, C, E and G ) compared to control brain regions ( B, D, F and H ) using long-pulse ( A, B, E and F ) and RaSP ( C, D, G and H ) sequences. The total count of CD3 + ( I ) and MHC class II + ( J ) cells was determined in treated brain regions targeted with ultrasound at 0.71 MPa with both long pulse and rapid short-pulse sequences compared to control (untreated) regions. A significant increase in T cells ( I ) and MHC class II + cells ( J ) was observed in ultrasound-treated compared to control brain regions in long-pulse treated brains. Scale bars represent 200 µm. The plot shows mean ± SEM (n= 3); * p-value ≤ 0.05 (0.0021 ( I )) and *** p-value ≤ 0.0001 (0.0001 ( J )).

Article Snippet: T cells were stained using a rat anti-mouse CD3 primary antibody (1:200; clone: KT3, #MCA500G, Bio-Rad, CA, USA) with 1% (w/v) milk powder (Tesco, Hertfordshire, England) dissolved in TBS (#524750-1EA, EMD Millipore Corporation, Burlington, MA, USA) and a secondary Alexa Fluor 488 goat anti-rat IgG H&L secondary antibody (1:400; #ab150157, Abcam, Cambridge, England) with 1% (w/v) milk powder in TBS.

Techniques: Immunofluorescence, Staining, Control

Journal: Translational Cancer Research

Article Title: OX40L and IL-2 combination strategy for gastric cancer immunotherapy

doi: 10.21037/tcr-2025-707

Figure Lengend Snippet: Immunohistochemical and flow cytometric analysis of gastric cancer tissue. (A) H&E histochemical staining of tumor-free area (left), tumor margin (middle), and gastric tumor site (right). (B) Representative IHC images for CD3 + , CD4 + , CD8 + , FOXP3, OX40, and OX40L in tumor-free area (left column), tumor margin (middle column), and gastric tumor site (right column) sections. (C) Immunofluorescence staining for the co-expression of CD3 + and OX40 in gastric cancer with anti-CD3 and anti-OX40 antibodies and DAPI for nuclear staining. (D) Flow cytometry analysis of OX40 expression in CD3 cells obtained from PBMC of gastric cancer patients (upper panel) and OX40 expression on CD3 on TILs in gastric cancer patients (low panel). Statistical analysis of all data were performed using one-way ANOVA. Data are shown as mean ± standard deviation. ns, nonsignificant; *, P<0.05; ***, P<0.001. ANOVA, analysis of variance; DAPI, 4',6-diamidino-2-phenylindole; H&E, hematoxylin & eosin; IHC, immunohistochemistry; OX40L, OX40 ligand; PBMC, peripheral blood mononuclear cells; TILs, tumor-infiltrating lymphocytes.

Article Snippet: Immunohistochemistry (IHC) analyses were performed using specific primary rabbit anti-human CD3 monoclonal antibody (Proteintech Group, Wuhan, China), mouse anti-human OX40 and rabbit anti-human OX40L monoclonal antibodies (CST, Danvers, MA, USA), and rabbit anti-human CD4 (Proteintech Group, Wuhan, China), mouse anti-human CD8 (Proteintech Group, Wuhan, China) antibody, rabbit anti-human FOXP3 monoclonal antibodies (CST, Danvers, MA, USA).

Techniques: Immunohistochemical staining, Staining, Immunofluorescence, Expressing, Flow Cytometry, Standard Deviation, Immunohistochemistry